Jiangsu Province Engineering Research Center of Novel Tumor-targeting Drug Conjugates

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology

Chaoyang Chen^1,2, Huajun Yang^1,2, Xiaoxia Ji³, Cheng Li¹, Mengting Yang¹, Lin Ge¹, Mengting Sun¹

¹Department of Medical Science and Technology, Suzhou Chien-shiung Institute of Technology, ²Jiangsu Province Engineering Research Center of Novel Tumor-targeting Drug Conjugates, ³Crown Bioscience Inc.

This article presents a simplified method for preparing 4 hemagglutination units antigen for Newcastle disease virus serological testing. By accurately determining the hemagglutination titer of the antigen, combined with a more rigorous back-titration method, and a well-defined adjustment process, it enhances test efficiency, reduces false positives, and improves disease surveillance in poultry.