Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus SerologyChaoyang Chen 1,2, Huajun Yang 1,2, Xiaoxia Ji 3, Cheng Li 1, Mengting Yang 1, Lin Ge 1, Mengting Sun 1
1Department of Medical Science and Technology, Suzhou Chien-shiung Institute of Technology, 2Jiangsu Province Engineering Research Center of Novel Tumor-targeting Drug Conjugates, 3Crown Bioscience Inc.
This article presents a simplified method for preparing 4 hemagglutination units antigen for Newcastle disease virus serological testing. By accurately determining the hemagglutination titer of the antigen, combined with a more rigorous back-titration method, and a well-defined adjustment process, it enhances test efficiency, reduces false positives, and improves disease surveillance in poultry.
