Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker DiscoveryGabriel Jakob Trauner *1, Dominika Bernath-Nagy *1,2, Melek Sükran Kalinyaprak 1, Sabine Merker 3, Marcin Luzarowski 3, Florian Leuschner 1,4, Norbert Frey 1,4, Evangelos Giannitsis 1, Jona Benjamin Krohn 1,4
1Department of Cardiology, Angiology and Pulmonology, University Hospital Heidelberg, 2Heart and Vascular Center, Semmelweis University, 3Core Facility for Mass Spectrometry and Proteomics, Centre for Molecular Biology at Heidelberg University (ZMBH), 4German Centre for Cardiovascular Research (DZHK), Partner site Heidelberg/Mannheim
This article describes a methodology for the isolation, characterization, and quantification of human plasma-derived extracellular vesicles (EV) and presents a workflow for label-free analysis of the EV proteome using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
