Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
299 Views
•
05:30 min
•
January 31st, 2025
DOI :
January 31st, 2025
•Gabriel Jakob Trauner*1, Dominika Bernath-Nagy*1,2, Melek Sükran Kalinyaprak1, Sabine Merker3, Marcin Luzarowski3, Florian Leuschner1,4, Norbert Frey1,4, Evangelos Giannitsis1, Jona Benjamin Krohn1,4
1Department of Cardiology, Angiology and Pulmonology, University Hospital Heidelberg, 2Heart and Vascular Center, Semmelweis University, 3Core Facility for Mass Spectrometry and Proteomics, Centre for Molecular Biology at Heidelberg University (ZMBH), 4German Centre for Cardiovascular Research (DZHK), Partner site Heidelberg/Mannheim
Chapters in this video
0:00
Introduction
2:04
Nanoparticle Tracking Analysis to Study Plasma Extracellular Vesicles and Protein Cargo for Cardiovascular Biomarker Research
4:51
Representative Results
Related Videos
09:22
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
16.4K Views
09:28
Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis
2.8K Views
09:18
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
9.4K Views
10:09
Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
6.8K Views
06:03
Isolation and Characterization of Microvesicles from Peripheral Blood
22.9K Views
08:32
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
13.6K Views
Isolation and Characterization of Human Adipocyte-Derived Extracellular Vesicles using Filtration and Ultracentrifugation
2.3K Views
12:27
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
4.5K Views
09:57
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
1.9K Views
02:36
Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles
867 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved