In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural ChangesJorge M. S. Faria 1,2, Pedro Barbosa 3,4, A. Cristina Figueiredo 4, Manuel Mota 3, Cláudia S. L. Vicente 3
1INIAV, I.P., National Institute for Agrarian and Veterinary Research, 2GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB NOVA), 3MED – Mediterranean Institute for Agriculture, Environment and Development & CHANGE – Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de Évora, 4Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa
The protocol describes infection of Solanum tuberosum roots with plant parasitic nematodes under in vivo greenhouse conditions and potato in vitro transgenic roots for histochemical analysis of root structure through optical microscopy.
