Extraction and Identification of Micronuclei from Human Peripheral Blood LymphocytesPing Wu 1, Zhengxi Tian 1, Lei Xie 1, Jiang Yao 2, Qijie Li 2
1Department of Occupational Diseases, The Second Affiliated Hospital of Chengdu Medical College Nuclear Industry 416 Hospital, 2Medicine and Engineering Interdisciplinary Research Laboratory of Nursing & Materials, West China Hospital, Sichuan University/West China School of Nursing
This protocol outlines a method for extracting and purifying micronuclei from human lymphocytes using sucrose density gradient centrifugation. It provides an experimental basis for investigating the composition and function of micronuclei.
