Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line MonitoringTina Vodopivec Seravalli *1, Janja Skok *1, Tjaša Marušič 2, Rok Sekirnik 1
1Sartorius BIA Separations d.o.o., a Sartorius company, 2Biotechnical Faculty, University of Ljubljana
Optimizing in vitro transcription (IVT) is critical for cost-effective mRNA production. This protocol details an analytical chromatographic method for at-line analysis of IVT reaction, monitoring NTP depletion and mRNA production in batch or fed-batch modes, applicable to various RNA modalities, enhancing productivity and reducing costs.
