Sartorius BIA Separations d.o.o., a Sartorius company

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Optimization of In vitro Transcription Reaction for mRNA Production Using Chromatographic At-Line Monitoring

Tina Vodopivec Seravalli^*1, Janja Skok^*1, Tjaša Marušič², Rok Sekirnik¹

¹Sartorius BIA Separations d.o.o., a Sartorius company, ²Biotechnical Faculty, University of Ljubljana

Optimizing in vitro transcription (IVT) is critical for cost-effective mRNA production. This protocol details an analytical chromatographic method for at-line analysis of IVT reaction, monitoring NTP depletion and mRNA production in batch or fed-batch modes, applicable to various RNA modalities, enhancing productivity and reducing costs.