Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian CellsTimo Appelhans 1, Felix R.M. Beinlich 1, Christian P. Richter 1, Rainer Kurre 2, Karin B. Busch 1,3
1School of Biology, University of Osnabrück, 2Center of Cellular Nanoanalytics, Integrated Bioimaging Facility, University of Osnabrück, 3Department of Biology, WWU Münster
Here, we present a protocol for multi-color localization of single membrane proteins in organelles of live cells. To attach fluorophores, self-labeling proteins are used. Proteins, located in different membranes compartments of the same organelle, can be localized with a precision of ~18 nm.
