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University of Osnabrück

1 ARTICLES PUBLISHED IN JoVE

Biology

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Timo Appelhans¹, Felix R.M. Beinlich¹, Christian P. Richter¹, Rainer Kurre², Karin B. Busch^1,3

¹School of Biology, University of Osnabrück, ²Center of Cellular Nanoanalytics, Integrated Bioimaging Facility, University of Osnabrück, ³Department of Biology, WWU Münster

Here, we present a protocol for multi-color localization of single membrane proteins in organelles of live cells. To attach fluorophores, self-labeling proteins are used. Proteins, located in different membranes compartments of the same organelle, can be localized with a precision of ~18 nm.