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University of Trento

8 ARTICLES PUBLISHED IN JoVE

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Bioengineering

The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
Amy C. Spencer 1, Paola Torre 1, Sheref S. Mansy 1
1Centre for Integrative Biology, University of Trento

Herein we describe simple methods for the preparation of vesicles, the encapsulation of transcription and translation machinery, and the monitoring of protein production. The resulting cell-free systems can be used as a starting point from which to build increasingly complex cellular mimics.

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Biology

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Viktoryia Sidarovich 1, Valentina Adami 2, Alessandro Quattrone 1
1Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, 2High Throughput Screening Core Facility, Centre for Integrative Biology, University of Trento

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

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Neuroscience

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
Giovanni Provenzano 1, Luca Pangrazzi 1, Andrea Poli 2, Nicoletta Berardi 2, Yuri Bozzi 1,2
1Centre for Integrative Biology (CIBIO), University of Trento, 2CNR Neuroscience Institute, Pisa, Italy

We describe an immunohistochemistry protocol to study the profile of hippocampal neuron activation following exposure to a spatial learning task in a mouse model characterized by cognitive deficits of neurodevelopmental origin. This protocol can be applied to both genetic or pharmacological mouse models characterized by cognitive deficits.

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Genetics

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Silvia Dal Santo *1, Mauro Commisso *1, Erica D'Incà 1, Andrea Anesi 1,2, Matteo Stocchero 3, Sara Zenoni 1, Stefania Ceoldo 1, Giovanni B. Tornielli 1, Mario Pezzotti 1, Flavia Guzzo 1
1Biotechnology Department, University of Verona, 2Lab. of Bioorganic Chemistry, Physics Department, University of Trento, 3S-IN Soluzioni Informatiche

This article describes the application of untargeted metabolomics, transcriptomics and multivariate statistical analysis to grape berry transcripts and metabolites in order to gain insight into the terroir concept, i.e., the impact of the environment on berry quality traits.

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JoVE Core

Non-equilibrium Microwave Plasma for Efficient High Temperature Chemistry
Dirk van den Bekerom 1, Niek den Harder 1, Teofil Minea 1, Nicola Gatti 1,2, Jose Palomares Linares 1, Waldo Bongers 1, Richard van de Sanden 1,3, Gerard van Rooij 1,3
1Dutch Institute for Fundamental Energy Research, 2University of Trento, 3Eindhoven University of Technology

This article describes a flowing microwave reactor that is used to drive efficient non-equilibrium chemistry for the application of conversion/activation of stable molecules such as CO2, N2 and CH4. The goal of the procedure described here is to measure the in situ gas temperature and gas conversion.

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Genetics

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
Laura Avogaro 1, Claudio Oss Pegorar 1, Nicole Bettin 1,2, Emilio Cusanelli 1
1Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, 2Department of Life Sciences, University of Trieste

Here, we present a protocol to generate cancer cell clones containing a MS2 sequence tag at a single subtelomere. This approach, relying on the MS2-GFP system, enables visualization of the endogenous transcripts of telomeric repeat-containing RNA (TERRA) expressed from a single telomere in living cells.

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Cancer Research

Yeast As a Chassis for Developing Functional Assays to Study Human P53
Paola Monti 1, Bartolomeo Bosco 2, Sara Gomes 3, Lucilia Saraiva *3, Gilberto Fronza *1, Alberto Inga *2
1Mutagenesis and Cancer Prevention Unit, Ospedale Policlinico San Martino, 2Department CIBIO, University of Trento, 3LAQV/REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto

Presented here are four protocols to construct and exploit yeast Saccharomyces cerevisiae reporter strains to study human P53 transactivation potential, impacts of its various cancer-associated mutations, co-expressed interacting proteins, and the effects of specific small molecules.

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Biology

Methods for Rearing the Parasitoid Ganaspis brasiliensis, a Promising Biological Control Agent for the Invasive Drosophila suzukii
Marco Valerio Rossi-Stacconi 1, Xingeng Wang 2, Amanda Stout 2, Lorenzo Fellin 1,3, Kent M. Daane 4, Antonio Biondi 5, Judith M. Stahl 4, Matthew L. Buffington 6, Gianfranco Anfora 1,3, Kim A. Hoelmer 2
1Research and Innovation Centre, Fondazione Edmund Mach, 2Beneficial Insects Introduction Research Unit, Agricultural Research Service, United States Department of Agriculture, 3Center for Agriculture, Food and Environment, University of Trento, 4Department of Environmental Science, Policy and Management, University of California Berkeley, 5Department of Agriculture, Food and Environment, University of Catania, 6Systematic Entomology Laboratory, Agricultural Research Service, United States Department of Agriculture

Ganaspis brasiliensis-a larval parasitoid of Drosophila suzukii (a global invasive fruit crop pest)-has been approved or is considered for introduction into Europe and the United States for biological control of this pest. This article provides protocols for both small-scale and large-scale rearing of this parasitoid.

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