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Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells

7.3K Views

09:13 min

January 17th, 2019

DOI :

10.3791/58790-v

January 17th, 2019


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Cancer Cell Clones

Chapters in this video

0:04

Title

0:37

Selection of Neomycin Resistant Clones

3:41

Screening of Neomycin Resistant Clones

5:34

Elimination of the Neomycin Resistance Gene from the MS2 Cassette Integrated at Subtelomere 15q

6:06

Visualization of TERRA-MS2 Transcripts in Living Cells

6:49

Results: Cancer Cell Clones with MS2 Sequence Tag Created and Imaged

8:17

Conclusion

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