chinese academy of forestry

5 ARTICLES PUBLISHED IN JoVE

Biology

Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus

Najie Zhu^*1, Liqun Bai^*1, Stefan Schütz², Baojun Liu³, Zhenyu Liu³, Xingyao Zhang⁴, Hongshi Yu¹, Jiafu Hu^1,5

¹Department of Forest Protection, Zhejiang Agricultural & Forestry University, ²Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, ³College of Plant Protection, Shandong Agricultural University, ⁴Institute of Forest Protection, Chinese Academy of Forestry, ⁵Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University

A protocol for investigating the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, is presented. Behavioral features of B. xylophilus are described in the mating process.

Biology

Development of an Individual-Tree Basal Area Increment Model using a Linear Mixed-Effects Approach

Wenwen Wang¹, Yanfeng Bai², Chunqian Jiang², Jinghui Meng¹

¹Research Center of Forest Management Engineering of National Forestry and Grassland Administration, Beijing Forestry University, ²Research Institute of Forestry, Chinese Academy of Forestry

Mixed-effects models are flexible and useful tools for analyzing data with a hierarchical stochastic structure in forestry and could also be used to significantly improve the performance of forest growth models. Here, a protocol is presented that synthesizes information relating to linear mixed-effects models.

Biology

In Vivo Leaf Inoculation: An Alternative Method to Assess the Disease Resistance of Hybrid Clones in Poplar Breeding of Stem Canker Disease

Yutian Suo^*1,2, Yinan Zhang^*1,2, Zheng Li^1,2, Mingzhuo Zhao³, Wanna Shen^1,2, Yuchen Fu^1,2, Donglin Wang^1,2, Long Pan^1,2, Xiaohua Su⁴, Jiaping Zhao^1,2

¹State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, ²Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, ³Beijing Haidian Kaiwen Academy High School, ⁴State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry

We provide a step-by-step protocol for assessing poplar resistance to stem canker pathogens using an in vivo leaf inoculation method. This method is especially suitable for large-scale assessment of Cytospora chrysosperma and Botryosphaeria dothidea canker disease resistance in poplar breeding progeny in China.

Biology

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Min Li^*1,2, Donglin Wang^*1,2, Yuchen Fu^1,2, Mingzhuo Zhao³, Zheng Li^1,2, Wanna Shen^1,2, Long Pan^1,2, Xiaohua Su⁴, Jiaping Zhao^1,2

Here, we present a protocol to induce the production of adventitious roots (ARs) through the phloem- or epidermis-girdle fungal pathogen inoculation pathway, which is suitable for the study of root biology and the light response-related physiological processes in poplar.

Biology

Inducing the Entry of Third Stage Dispersal Juveniles of Bursaphelenchus xylophilus into Cryptobiosis through Osmotic Regulation

Long Pan^1,2,3, Donglin Wang^1,2,3, Yutian Suo^1,2,3, Yaqi Wu^1,2,3, Wenyi Zhao⁴, Jian Chen⁵, Zhen Yu⁵, Jiaping Zhao^1,2,3

¹1State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, ²Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, ³Tianjin Institute of Forestry Science, Chinese Academy of Forestry, ⁴Ningbo Yongsen Biotechnology Co. Ltd, ⁵Xiangshan Natural Resources and Planning Bureau of Zhejiang

The present protocol describes a method for inducing the dispersal of third-stage juveniles (pre-dauer juveniles, J_Ⅲ) of Bursaphelenchus xylophilus into cryptobiosis through osmotic regulation of potassium chloride (KCl) ions. This method can provide technical support for research on the stress resistance mechanisms of B. xylophilus.