University of Essex
2 ARTICLES PUBLISHED IN JoVE
Environment
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Enrico Tatti 1, Boyd A. McKew 2, Corrine Whitby 2, Cindy J. Smith 1
1Microbiology, School of Natural Sciences, National University of Ireland Galway, 2School of Biological Sciences, University of Essex
A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.
Biochemistry
Fixed Target Serial Data Collection at Diamond Light Source
Sam Horrell 1, Danny Axford 1, Nicholas E. Devenish 1, Ali Ebrahim 1, Michael A. Hough 2, Darren A. Sherrell 1,3, Selina L. S. Storm 1,4, Ivo Tews 5, Jonathan A. R. Worrall 2, Robin L. Owen 1
1Diamond Light Source, Harwell Science and Innovation Campus, 2School of Life Sciences, University of Essex, 3X-ray Science Division, Argonne National Laboratory, 4European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, 5Biological Sciences, Institute for Life Sciences, University of Southampton
We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24.
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