Accedi

University of Essex

2 ARTICLES PUBLISHED IN JoVE

Environment

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Enrico Tatti¹, Boyd A. McKew², Corrine Whitby², Cindy J. Smith¹

¹Microbiology, School of Natural Sciences, National University of Ireland Galway, ²School of Biological Sciences, University of Essex

A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.

Biochemistry

Fixed Target Serial Data Collection at Diamond Light Source

Sam Horrell¹, Danny Axford¹, Nicholas E. Devenish¹, Ali Ebrahim¹, Michael A. Hough², Darren A. Sherrell^1,3, Selina L. S. Storm^1,4, Ivo Tews⁵, Jonathan A. R. Worrall², Robin L. Owen¹

¹Diamond Light Source, Harwell Science and Innovation Campus, ²School of Life Sciences, University of Essex, ³X-ray Science Division, Argonne National Laboratory, ⁴European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, ⁵Biological Sciences, Institute for Life Sciences, University of Southampton

We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24.