University of Muenster

8 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Myelin Oligodendrocyte Glycoprotein (MOG_35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

Stefan Bittner^1,2, Ali M. Afzali¹, Heinz Wiendl¹, Sven G. Meuth^1,3

¹Department of Neurology, University of Münster, ²Interdisciplinary Center for Clinical Research (IZKF), Münster, ³Institute of Physiology – Neuropathophysiology, University of Münster

Experimental autoimmune encephalomyelitis (EAE) is an established animal model of multiple sclerosis. C57BL/6 mice are immunized with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 (MOG_35-55), resulting in an ascending flaccid paralysis caused by autoreactive immune cells in the central nervous system. Protocols for disease induction and monitoring will be discussed.

Neuroscience

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Tobias Ruck¹, Stefan Bittner^1,2, Lisa Epping¹, Alexander M. Herrmann¹, Sven G. Meuth^1,3

¹Department of Neurology, University of Münster, ²Interdisciplinary Center for Clinical Research (IZKF) Münster, ³Institute of Physiology I — Neuropathophysiology I, University of Münster

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

Immunology and Infection

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

Kerstin Göbel¹, Stefan Bittner^1,2, Manuela Cerina³, Alexander M. Herrmann¹, Heinz Wiendl¹, Sven G. Meuth^1,3

¹Department of Neurology, University of Münster, ²Germany and Interdisciplinary Center for Clinical Research (IZKF) Münster, ³Institute of Physiology I - Neuropathophysiology I, University of Münster

To address mechanisms of demyelination and neuronal apoptosis in cortical lesions of inflammatory demyelinating disorders, different animal models are used. We here describe an ex vivo approach by using oligodendrocyte-specific CD8⁺ T-cells on brain slices, resulting in oligodendroglial and neuronal death. Potential applications and limitations of the model are discussed.

Behavior

Neuro-rehabilitation Approach for Sudden Sensorineural Hearing Loss

Kenichi Sekiya^1,2, Munehisa Fukushima³, Henning Teismann^4,5, Lothar Lagemann⁴, Ryusuke Kakigi^1,6, Christo Pantev⁴, Hidehiko Okamoto^1,4,6

¹Department of Integrative Physiology, National Institute for Physiological Sciences, ²Department of Otolaryngology, Head and Neck Surgery, Nagoya City University Graduate School of Medical Sciences and Medical School, ³Department of Otorhinolaryngology, Kansai Rosai Hospital, ⁴Institute for Biomagnetism and Biosignalanalysis, University of Muenster, ⁵Institute for Epidemiology and Social Medicine, University of Muenster, ⁶Sokendai Graduate University for Advanced Studies

This article reports the development of a neuro-rehabilitation approach, "constraint-induced sound therapy (CIST)" for sudden sensorineural hearing loss. The aim of CIST is to prevent maladaptive cortical reorganization by using an enriched acoustic environment. CIST represents a safe, easy, inexpensive, and effective approach to treat sudden sensorineural hearing loss.

Immunology and Infection

An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction

Ivan Kuzmanov¹, Alexander M. Herrmann¹, Hans-Joachim Galla², Sven G. Meuth¹, Heinz Wiendl^*1, Luisa Klotz^*1

¹Department of Neurology, University Hospital Münster, ²Institute of Biochemistry, University of Münster

Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.

Biology

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Tanumoy Saha¹, Isabel Rathmann¹, Milos Galic¹

¹DFG Cluster of Excellence 'Cells in Motion', (EXC 1003), Institute of Medical Physics and Biophysics, University of Muenster

We present a software solution for semi-automated tracking of relative protein concentration along the length of dynamic cellular protrusions.

Neuroscience

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

Thomas Müntefering^*1, Alexander P.E. Michels^*1, Steffen Pfeuffer¹, Sven G. Meuth^*1, Tobias Ruck^*1

¹Institute for Translational Neurology and Neurology Clinic, University of Muenster

Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".

Biology

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Anthony L. Hessel^1,4, Daniel Hahn^1,2, Markus de Marées³

¹Faculty of Sport Science, Human Movement Science, Ruhr University Bochum, ²School of Human Movement and Nutrition Sciences, The University of Queensland, ³Faculty of Sport Science, Sports Medicine and Sports Nutrition, Ruhr University Bochum, ⁴Institute of Physiology II, University of Muenster

This technical report describes a variation of the modified Bergström technique for the biopsy of the musculus tibialis anterior that limits fiber damage.