Translational Genomics Research Institute

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

Pawan Noel¹, Ruben Muñoz¹, George W. Rogers², Andrew Neilson², Daniel D. Von Hoff¹, Haiyong Han¹

¹Molecular Medicine Division, Translational Genomics Research Institute, ²Agilent Technologies

Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.