Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and FibroblastsPawan Noel 1, Ruben Muñoz 1, George W. Rogers 2, Andrew Neilson 2, Daniel D. Von Hoff 1, Haiyong Han 1
1Molecular Medicine Division, Translational Genomics Research Institute, 2Agilent Technologies
Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.
