Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
35.2K Views
•
09:51 min
•
March 5th, 2013
DOI :
March 5th, 2013
•Chapters in this video
0:05
Title
1:22
Generation of Cell Lines Stably Expressing TetR
4:51
pTER and pT-Rex DEST30: shRNA or cDNAs Expression
5:30
Stable Cell Lines with Tetracycline-inducible Expression
8:31
Results: Stable Human Tet-on Cell Lines
9:27
Conclusion
Related Videos
10:00
Controllable Ion Channel Expression through Inducible Transient Transfection
9.3K Views
10:59
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
12.3K Views
09:24
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
4.3K Views
16:04
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
14.5K Views
08:51
Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
9.1K Views
08:48
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
8.4K Views
11:21
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
9.4K Views
13:54
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
10.9K Views
08:27
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
12.3K Views
02:22
Inducible Tet-On Regulatable System-Based Gene Expression In Vivo: A Tetracycline-Induced Gene Expression System for Target Gene Transcription Activation in Mouse Model
2.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved