Waseda University

10 ARTICLES PUBLISHED IN JoVE

Neuroscience

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo

Takafumi Inoue¹, Keishi Narita², Yuta Nonami¹, Hideki Nakamura¹, Sen Takeda²

¹Department of Life Science and Medical Bioscience, Faculty of Science and Engineering, Waseda University, ²Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine & Engineering, University of Yamanashi

In this study, a detailed light microscopic technique was optimized for real-time observation and analysis of the motion of CPEC cilia ex vivo together with an electron microscopic method for ultrastructural analysis.

Engineering

Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores

Sherif A. El-Safty^1,2, Mohamed A. Shenashen¹, Masaru Sakai³, Emad Elshehy¹, Kohmei Halada¹

¹National Institute for Materials Science, Japan, ²Graduate School for Advanced Science and Engineering, Waseda University, ³Center for Research in Isotopes and Environmental Dynamics, Tsukuba University

Because of the importance and extensive use of palladium, gold and cobalt metals in high-technology equipment, their recovery and recycling constitute an important industrial challenge. The metal recovery system described herein is a simple, low-cost means for the effective detection, removal, and recovery of these metals from the urban mine.

Bioengineering

Photopatterning Proteins and Cells in Aqueous Environment Using TiO₂ Photocatalysis

Hideaki Yamamoto^1,2, Takanori Demura³, Kohei Sekine³, Sho Kono³, Michio Niwano^2,4, Ayumi Hirano-Iwata^2,5, Takashi Tanii⁶

¹Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, ²CREST, Japan Science and Technology Agency, ³School of Fundamental Science and Engineering, Waseda University, ⁴Research Institute of Electrical Communication, Tohoku University, ⁵Graduate School of Biomedical Engineering, Tohoku University, ⁶Faculty of Science and Engineering, Waseda University

We describe a protocol for modifying cell affinity of a scaffold surface in aqueous environment. The method takes advantage of titanium dioxide photocatalysis to decompose organic film in the photo-irradiated region. We show that it can be used to create microdomains of scaffolding proteins, both ex situ and in situ.

Engineering

Fabrication of White Light-emitting Electrochemical Cells with Stable Emission from Exciplexes

Soichi Uchida¹, Daisuke Takizawa², Satoru Ikeda¹, Hironori Takeuchi³, Suzushi Nishimura¹, Hiroyuki Nishide², Yoshinori Nishikitani²

¹Specialty Chemicals & Materials Company, JX Nippon Oil & Energy Corporation, ²Department of Advanced Science and Engineering, Waseda University, ³Central Technical Research Laboratory, JX Nippon Oil & Energy Corporation

The authors present a method for fabricating stable white-light-emitting electrochemical cells utilizing emission from exciplexes formed between a blue-emitting fluorene polymer and aromatic amines.

Medicine

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

Kazuhiro Ogai¹, Masakazu Fukuoka², Kei-ichiro Kitamura³, Kiyoshi Uchide⁴, Tetsu Nemoto³

¹Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, ²Advanced Research Center for Human Sciences, Waseda University, ³Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, ⁴Asanogawa General Hospital

Recently, we developed a small wireless device for perspiration monitoring. In this article, we present detailed protocols on how to use the device for perspiration monitoring with an example of the sympathetic activity test.

Behavior

Observing the Transformation of Bodily Self-consciousness in the Squeeze-machine Experiment

Mai Minoura¹, Iori Tani², Takahiro Ishii³, Yukio- Pegio Gunji¹

¹Department of Intermedia Art and Science, Waseda University, ²Research Center for Kansei Value Creation, School of Science and Technology, Kwansei Gakuin University, ³Department of Occupational Therapy, Teikyo University of Science

The squeeze machine, a V-shaped device that provides pressure along the whole body is used in therapy especially for people with autism. Here, we focus on its possible use in experiments to capture the typical transformations of bodily self-consciousness with peri-personal space measurement.

Immunology and Infection

Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes

Naoyoshi Sakitani^*1, Takahiro Maekawa^*1, Kumiko Saitou^1,2, Katsuhiko Suzuki³, Shuhei Murase^1,4, Masakuni Tokunaga¹, Daisuke Yoshino⁵, Keisuke Sawada⁶, Atsushi Takashima⁷, Motoshi Nagao¹, Toru Ogata¹, Yasuhiro Sawada^1,8

¹Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities, ²Graduate School of Sport Sciences, Waseda University, ³Faculty of Sport Sciences, Waseda University, ⁴Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Tokyo, ⁵Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, ⁶University of Cincinnati College of Medicine, ⁷Department of Assistive Technology, National Rehabilitation Center for Persons with Disabilities, ⁸Department of Clinical Research, National Rehabilitation Center for Persons with Disabilities

Here we describe the protocols for applying defined mechanical loads to mouse calves and for monitoring the concomitant intramuscular pressure changes. The experimental systems that we have developed can be useful for investigating the mechanism behind the beneficial effects of physical exercise and massage.

Immunology and Infection

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

Masamune Morita¹, Yuri Ota^1,2, Kaoru Katoh¹, Naohiro Noda^1,2

¹Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), ²Department of Life Science and Medical Bioscience, Waseda University

We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.

Chemistry

Organic Structure-directing Agent-free Synthesis for *BEA-type Zeolite Membrane

Motomu Sakai¹, Noriyuki Yasuda², Yuto Tsuzuki², Masahiko Matsukata^1,2,3

¹Research Organization for Nano & Life Innovation, Waseda University, ²Department of Applied Chemistry, Waseda University, ³Advanced Research Institute for Science and Engineering, Waseda University

A *BEA seed crystal was loaded on a porous α-Al₂O₃ support by the dip-coating method, and hydrothermally grown without using an organic structure-directing agent. A *BEA-type zeolite membrane having very few defects was successfully prepared by the secondary growth method.

Behavior

Virtual Hand with Ambiguous Movement between the Self and Other Origin: Sense of Ownership and 'Other-Produced' Agency

Mai Minoura¹, Kei Kojima¹, Shuusaku Nomura², Yuta Nishiyama², Takashi Kawai¹, Yukio-Pegio Gunji¹

¹Department of Intermedia, Art and Science, School of Fundamental Science and Technology, Waseda University, ²Human Informatics Group, Graduate School of Information and Management Systems Engineering, Nagaoka University of Technology

While previous research on bodily self-consciousness assumed that self- and other-origin movements were perceptually distinguishable, this protocol allows them to be ambiguous on a virtual hand with unintentional slight movements. This enables us to observe one’s experience formed by SoO and other-produced SoA, rather than the absence of SoA.