JoVE Logo

Sign In

University of Adelaide

6 ARTICLES PUBLISHED IN JoVE

image

Biology

Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction
Avdesh Avdesh *1,2, Mengqi Chen *1,3, Mathew T. Martin-Iverson 1,2,4, Alinda Mondal 1,3, Daniel Ong 1, Stephanie Rainey-Smith 1,3, Kevin Taddei 1,3, Michael Lardelli 5, David M. Groth 6, Giuseppe Verdile 1,3, Ralph N. Martins 1,2,3,7
1Centre of Excellence for Alzheimer's Disease Research and Care, School of Medical sciences, Edith Cowan University, 2Centre for Clinical Research in Neuropsychiatry, Graylands Hospital, University of Western Australia, 3McCusker Alzheimer's Research foundation, 4School of Medicine and Pharmacology, University of Western Australia , 5Department of Molecular and Biomedical Sciences, University of Adelaide, 6School of Biomedical Sciences, Curtin University of Technology, 7School of Psychiatry and Clinical Neurosciences, University of Western Australia

This protocol outlines regular maintenance and care to maintain optimal conditions for zebrafish husbandry. The video illustrates the protocol for system maintenance, regular housing, feeding, breeding, and raising of zebrafish larvae.

image

Neuroscience

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
Sara Nullens 1, Annemie Deiteren 2, Wen Jiang 3, Christopher Keating 4, Hannah Ceuleers 1, Sven Francque 5, David Grundy 3, Joris G. De Man 1, Benedicte Y. De Winter 1
1Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, 2Visceral Pain Group, Discipline of Medicine, University of Adelaide, 3Department of Biomedical Sciences, University of Sheffield, 4Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, 5Department of Gastroenterology and Hepatology, Antwerp University Hospital

Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.

image

Bioengineering

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Jianwei Chen *1,2,3, Ning Shao *1,2,3, Jiaying Hu *4, Rong Li 4, Yuanshou Zhu 1,2,3, Dabing Zhang 4,5, Shujuan Guo 1,2,3, Junhou Hui 6, Peng Liu 6, Litao Yang 4, Sheng-Ce Tao 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University

This protocol describes the fabrication of a small, ready-to-use cassette that can be applied for visual detection of multiple nucleic acids in a single, test that is easy to operate. In this approach, a capillary array was used for multiplex and highly efficient detection of GMO targets.

image

Biology

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Dawei Xu *1,2, Palash Chandra Mondol *1, Muhammad Uzair 1, Matthew R. Tucker 3, Dabing Zhang 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide

This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.

image

Cancer Research

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
Hiroki Kobayashi 1,2,3,4, Krystyna A. Gieniec 1,2, Jia Q. Ng 1,2, Jarrad Goyne 1,2, Tamsin R. M. Lannagan 1,2, Elaine M. Thomas 1,2, Georgette Radford 1,2, Tongtong Wang 1,2, Nobumi Suzuki 1,2,5, Mari Ichinose 1,2, Josephine A. Wright 2, Laura Vrbanac 1,2, Alastair D. Burt 6, Masahide Takahashi 3,4,7, Atsushi Enomoto 3, Daniel L. Worthley 2, Susan L. Woods 1,2
1Adelaide Medical School, University of Adelaide, 2South Australian Health and Medical Research Institute (SAHMRI), 3Department of Pathology, Nagoya University Graduate School of Medicine, 4Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, 5Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, 6Translational and Clinical Research Institute, Newcastle University, 7International Center for Cell and Gene Therapy, Fujita Health University

Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.

image

JoVE Core

Optical Coherence Tomography Based Biomechanical Fluid-Structure Interaction Analysis of Coronary Atherosclerosis Progression
Harry J. Carpenter 1, Mergen H. Ghayesh 1, Anthony C. Zander 1, Juanita L. Ottaway 2, Giuseppe Di Giovanni 3, Stephen J. Nicholls 4, Peter J. Psaltis 3,5,6
1School of Mechanical Engineering, University of Adelaide, 2South Australian Health and Medical Research Institute (SAHMRI), 3Vascular Research Centre, Lifelong Health Theme, South Australian Health and Medical Research Institute (SAHMRI), 4Monash Cardiovascular Research Centre, 5Adelaide Medical School, University of Adelaide, 6Department of Cardiology, Central Adelaide Local Health Network

There is a need to determine which atherosclerotic lesions will progress in the coronary vasculature to guide intervention before myocardial infarction occurs. This article outlines the biomechanical modeling of arteries from Optical Coherence Tomography using fluid-structure interaction techniques in a commercial finite element solver to help predict this progression.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved