Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

6.2K Views

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07:59 min

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September 3rd, 2021

DOI :

10.3791/62630-v

September 3rd, 2021

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Hiroki Kobayashi¹^,²^,³^,⁴, Krystyna A. Gieniec¹^,², Jia Q. Ng¹^,², Jarrad Goyne¹^,², Tamsin R. M. Lannagan¹^,², Elaine M. Thomas¹^,², Georgette Radford¹^,², Tongtong Wang¹^,², Nobumi Suzuki¹^,²^,⁵, Mari Ichinose¹^,², Josephine A. Wright², Laura Vrbanac¹^,², Alastair D. Burt⁶, Masahide Takahashi³^,⁴^,⁷, Atsushi Enomoto³, Daniel L. Worthley², Susan L. Woods¹^,²

¹Adelaide Medical School, University of Adelaide, ²South Australian Health and Medical Research Institute (SAHMRI), ³Department of Pathology, Nagoya University Graduate School of Medicine, ⁴Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, ⁵Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, ⁶Translational and Clinical Research Institute, Newcastle University, ⁷International Center for Cell and Gene Therapy, Fujita Health University

Summary

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Colorectal Cancer

Liver Metastasis

Portal Vein Injection

Tumor Microenvironment

CAS cancer associated Stroma

Therapeutic Strategies

Tumoroids

Surgical Procedure

Pain Management

Aseptic Surgical Area

Buprenorphine

Mouse Model

Visualization Techniques

Metastatic Cancer Cells

Chapters in this video

0:04

Introduction

1:18

Portal Vein Injection of Colorectal Cancer Organoids

5:39

Results: Liver Metastases, Tumor Growth Kinetics, Bone Morphogenetic Protein Signaling and Tumor Proliferation Induced by Portal Vein AAV8-Injection