An Antegrade Perfusion Method for Cardiomyocyte Isolation from MiceMariko Omatsu-Kanbe 1, Ryo Fukunaga 1, Xinya Mi 1, Hiroshi Matsuura 1
1Department of Physiology, Shiga University of Medical Science
We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.
