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Universidade Nova de Lisboa

6 ARTICLES PUBLISHED IN JoVE

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Medicine

A Model of Free Tissue Transfer: The Rat Epigastric Free Flap
Diogo Casal 1,4, Diogo Pais 1, Inês Iria 3,4, Eduarda Mota-Silva 5, Maria-Angélica Almeida 2, Sara Alves 6, Cláudia Pen 6, Ana Farinho 4, Luís Mascarenhas-Lemos 1,6, José Ferreira-Silva 6, Mário Ferraz-Oliveira 6, Valentina Vassilenko 5, Paula A. Videira 3,4, João Gory O'Neill 1,5
1Anatomy Department, NOVA Medical School, Universidade NOVA de Lisboa, 2Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central - Hospital de São José, 3UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 4CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, 5Physics Department, Faculty of Sciences and Technology, LIBPhys, 6Pathology Department, Centro Hospitalar de Lisboa Central – Hospital de São José

This paper describes the steps required to raise a fasciocutaneous epigastric free flap and transfer it to the neck in the rat.

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Medicine

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia
Augusto Ministro 1,2, Paula de Oliveira 1, Raquel J. Nunes 1, André dos Santos Rocha 1, Tiago Ferreira 2, J. Goyri-O'Neill 3, Susana Constantino Rosa Santos 1
1Centro Cardiovascular da Universidade de Lisboa, Lisbon School of Medicine of the Universidade de Lisboa, 2Centro Hospitalar Universitário Lisboa Norte, 3Nova Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa

Here, a critical hindlimb ischemia experimental model is presented followed by a battery of functional, histologic and molecular tests to assess the effectiveness of angiogenic therapies.​

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Medicine

Functional and Physiological Methods of Evaluating Median Nerve Regeneration in the Rat
Diogo Casal *1,2, Eduarda Mota-Silva *3, Inês Iria 4,5, Diogo Pais 1, Ana Farinho 6, Sara Alves 7, Cláudia Pen 7, Luís Mascarenhas-Lemos 7, José Ferreira-Silva 7, Mário Ferraz-Oliveira 7, Valentina Vassilenko 3, Paula A. Videira 4,5, João Goyri-O'Neill 1
1Anatomy Department, NOVA Medical School, 2Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central, 3Physics Department, Faculty of Sciences and Technology, LIBPhys, 4Glycoimmunology, CEDOC, NOVA Medical School, 5UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 6Tissue Repair Unit, CEDOC, NOVA Medical School, 7Pathology Department, Centro Hospitalar de Lisboa Central

Presented is a protocol to produce different types of median nerve (MN) lesions and repair in the rat. Additionally, the protocol shows how to evaluate the functional recovery of the nerve using several noninvasive behavioral tests and physiological measurements.

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Chemistry

Preparation of Binary and Ternary Deep Eutectic Systems
Liane Meneses 1, Filipa Santos 1, Ana Rita Gameiro 1, Alexandre Paiva 1, Ana Rita C. Duarte 1
1LAQV, REQUIMTE, Departamento de Química da Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa

This protocol aims to standardize the preparation of deep eutectic systems throughout the scientific community so that these systems can be reproduced.

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Neuroscience

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
Ana Filipa Isidro 1, Alexandra M. Medeiros 2, Isaura Martins 1, Dalila Neves-Silva 1, Leonor Saúde 1,3, César S. Mendes 2
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 2iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, NMS|FCM, Universidade Nova de Lisboa, 3Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa

An experimental pipeline to quantitatively describe the locomotor pattern of freely walking mice using the MouseWalker (MW) toolbox is provided, ranging from initial video recordings and tracking to post-quantification analysis. A spinal cord contusion injury model in mice is employed to demonstrate the usefulness of the MW system.

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Immunology and Infection

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes
Vanessa C. C. Luz 1,2, Zélia Silva 1,2, Patrícia Sobral 1,2,3, Ankit Tanwar 4,5, Rachel L. Paterson 6, Paula A. Videira 1,2,7
1Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology, Albert Einstein College of Medicine, 5Department of Oncology, Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, 7CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

A unique, comprehensive protocol to generate de-sialylated human monocyte-derived dendritic cells (mo-DCs) from isolated peripheral blood mononuclear cells (PBMCs) using a sialidase treatment is presented. Further, methods to assess the phenotypic and functional characterization of mo-DCs and evaluate how sialidase treatment improves the maturation level of mo-DCs are described.

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