Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

3.3K Views

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13:36 min

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October 20th, 2023

DOI :

10.3791/65525-v

October 20th, 2023

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Vanessa C. C. Luz¹^,², Zélia Silva¹^,², Patrícia Sobral¹^,²^,³, Ankit Tanwar⁴^,⁵, Rachel L. Paterson⁶, Paula A. Videira¹^,²^,⁷

¹Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, ²UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, ³LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, ⁴Department of Microbiology and Immunology, Albert Einstein College of Medicine, ⁵Department of Oncology, Albert Einstein College of Medicine, ⁶Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, ⁷CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

Summary

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Sialylated Glycans

Glycoimmunology

Monocyte derived Dendritic Cells

Glycan Profiling

Sialic Acid

Immune Response Modulation

Therapeutic Modulation

Enzymatic Treatment

Glycan Binding Proteins

Cancer Therapies

Congenital Disorders Of Glycosylation

Immunohistochemistry

Mass Spectrometry

Dendritic Cells Maturation

Chapters in this video

0:00

Introduction

3:09

Monocyte Isolation and Monocyte Differentiation into Monocyte‐Derived Dendritic Cells

9:24

Treatment of the Monocyte‐Derived Dendritic Cells (mo‐DC) with Sialidase

12:16

Maturation Profiling of Human Monocyte‐Derived Dendritic Cells (Mo‐DCs)

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