Universidade Nova de Lisboa

6 ARTICLES PUBLISHED IN JoVE

Medicine

A Model of Free Tissue Transfer: The Rat Epigastric Free Flap

Diogo Casal^1,4, Diogo Pais¹, Inês Iria^3,4, Eduarda Mota-Silva⁵, Maria-Angélica Almeida², Sara Alves⁶, Cláudia Pen⁶, Ana Farinho⁴, Luís Mascarenhas-Lemos^1,6, José Ferreira-Silva⁶, Mário Ferraz-Oliveira⁶, Valentina Vassilenko⁵, Paula A. Videira^3,4, João Gory O'Neill^1,5

¹Anatomy Department, NOVA Medical School, Universidade NOVA de Lisboa, ²Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central - Hospital de São José, ³UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, ⁴CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, ⁵Physics Department, Faculty of Sciences and Technology, LIBPhys, ⁶Pathology Department, Centro Hospitalar de Lisboa Central – Hospital de São José

This paper describes the steps required to raise a fasciocutaneous epigastric free flap and transfer it to the neck in the rat.

Medicine

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia

Augusto Ministro^1,2, Paula de Oliveira¹, Raquel J. Nunes¹, André dos Santos Rocha¹, Tiago Ferreira², J. Goyri-O'Neill³, Susana Constantino Rosa Santos¹

¹Centro Cardiovascular da Universidade de Lisboa, Lisbon School of Medicine of the Universidade de Lisboa, ²Centro Hospitalar Universitário Lisboa Norte, ³Nova Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa

Here, a critical hindlimb ischemia experimental model is presented followed by a battery of functional, histologic and molecular tests to assess the effectiveness of angiogenic therapies.

Medicine

Functional and Physiological Methods of Evaluating Median Nerve Regeneration in the Rat

Diogo Casal^*1,2, Eduarda Mota-Silva^*3, Inês Iria^4,5, Diogo Pais¹, Ana Farinho⁶, Sara Alves⁷, Cláudia Pen⁷, Luís Mascarenhas-Lemos⁷, José Ferreira-Silva⁷, Mário Ferraz-Oliveira⁷, Valentina Vassilenko³, Paula A. Videira^4,5, João Goyri-O'Neill¹

¹Anatomy Department, NOVA Medical School, ²Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central, ³Physics Department, Faculty of Sciences and Technology, LIBPhys, ⁴Glycoimmunology, CEDOC, NOVA Medical School, ⁵UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, ⁶Tissue Repair Unit, CEDOC, NOVA Medical School, ⁷Pathology Department, Centro Hospitalar de Lisboa Central

Presented is a protocol to produce different types of median nerve (MN) lesions and repair in the rat. Additionally, the protocol shows how to evaluate the functional recovery of the nerve using several noninvasive behavioral tests and physiological measurements.

Chemistry

Preparation of Binary and Ternary Deep Eutectic Systems

Liane Meneses¹, Filipa Santos¹, Ana Rita Gameiro¹, Alexandre Paiva¹, Ana Rita C. Duarte¹

¹LAQV, REQUIMTE, Departamento de Química da Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa

This protocol aims to standardize the preparation of deep eutectic systems throughout the scientific community so that these systems can be reproduced.

Neuroscience

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury

Ana Filipa Isidro¹, Alexandra M. Medeiros², Isaura Martins¹, Dalila Neves-Silva¹, Leonor Saúde^1,3, César S. Mendes²

¹Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, ²iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, NMS|FCM, Universidade Nova de Lisboa, ³Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa

An experimental pipeline to quantitatively describe the locomotor pattern of freely walking mice using the MouseWalker (MW) toolbox is provided, ranging from initial video recordings and tracking to post-quantification analysis. A spinal cord contusion injury model in mice is employed to demonstrate the usefulness of the MW system.

Immunology and Infection

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

Vanessa C. C. Luz^1,2, Zélia Silva^1,2, Patrícia Sobral^1,2,3, Ankit Tanwar^4,5, Rachel L. Paterson⁶, Paula A. Videira^1,2,7

¹Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, ²UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, ³LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, ⁴Department of Microbiology and Immunology, Albert Einstein College of Medicine, ⁵Department of Oncology, Albert Einstein College of Medicine, ⁶Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, ⁷CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

A unique, comprehensive protocol to generate de-sialylated human monocyte-derived dendritic cells (mo-DCs) from isolated peripheral blood mononuclear cells (PBMCs) using a sialidase treatment is presented. Further, methods to assess the phenotypic and functional characterization of mo-DCs and evaluate how sialidase treatment improves the maturation level of mo-DCs are described.