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university of the free state

5 ARTICLES PUBLISHED IN JoVE

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Environment

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
Charlotte Philippe 1,2, Arnout F. Gregoir 1, Eli S. J. Thoré 1, Gudrun De Boeck 2, Luc Brendonck 1,3, Tom Pinceel 1,4
1Animal Ecology, Global Change and Sustainable Development, University of Leuven, 2Systemic Physiological and Ecotoxicological Research, University of Antwerp, 3Water Research Group, Unit for Environmental Sciences and Management, North-West University, 4Centre for Environmental Management, University of the Free State

In this work, we describe an acute, chronic and multigenerational bioassay to study the effects of single and combined stressors on the Turquoise killifish Nothobranchius furzeri. This protocol is designed to study life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.

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Immunology and Infection

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
Uju L. Madu 1, Olihile M. Sebolai 1
1Department of Microbial, Biochemical and Food Biotechnology, University of the Free State

This paper details a protocol for preparing a co-culture of cryptococcal cells and amoebae that is studied using still, fluorescent images and high-resolution transmission electron microscope images. Illustrated here is how quantitative data can complement such qualitative information.

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Medicine

Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg
Christian Klopsch 1, Alexander Kaminski 1, Friedrich Prall 2, Pascal Dohmen 1,3
1Department of Cardiac Surgery, Heart Center Rostock, Rostock University Medical Center, University of Rostock, 2Institute of Pathology, Rostock University Medical Center, University of Rostock, 3Department of Cardiothoracic Surgery, Faculty of Health Science, University of the Free State

Presented here is a protocol for antegrade endoscopic vein harvesting from the lower leg, which can safely be introduced in routine coronary artery bypass grafting. Vein grafts present excellent graft quality following this standardized protocol with positioning of the legs, minimally invasive access to the vein, and antegrade endoscopic vein harvesting.

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Medicine

Radiation Planning Assistant - A Web-based Tool to Support High-quality Radiotherapy in Clinics with Limited Resources
Laurence Edward Court 1, Ajay Aggarwal 2, Hester Burger 3, Carlos Cardenas 4, Christine Chung 1, Raphael Douglas 1, Monique du Toit 5, Anuja Jhingran 1, Raymond Mumme 1, Sikudhani Muya 6, Komeela Naidoo 5, Jerry Ndumbalo 6, Tucker Netherton 1, Callistus Nguyen 1, Adenike Olanrewaju 1, Jeannette Parkes 3, Willie Shaw 7, Christoph Trauernicht 5, Melody Xu 8, Jinzhong Yang 1, Lifei Zhang 1, Hannah Simonds 9, Beth M. Beadle 10
1The University of Texas MD Anderson Cancer Center, 2Guy’s and St. Thomas’ Hospital, 3Groote Schuur Hospital and University of Cape Town, 4University of Alabama at Birmingham, 5Tygerberg Hospital and Stellenbosch University, 6Ocean Road Cancer Institute, 7University of the Free State, 8University of California-San Francisco, 9University Hospitals Plymouth NHS Trust, 10Stanford University

This protocol describes a series of automated tools designed for high-quality radiotherapy autocontouring and autoplanning that are being packaged into a web-based service to maximize robustness and scalability while minimizing operational costs.

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Biochemistry

Elucidating #946;-1,3-Glucanase and Peroxidase Physicochemical Properties of Wheat Cell Wall Defense Mechanism Against Diuraphis noxia Infestation
Siphephelo N.N. Zondo 1, Lintle Mohase 2, Vicki Tolmay 3, Mpho S. Mafa 1
1Carbohydrates and Enzymology Laboratory (CHEM-LAB), Department of Plant Sciences, University of the Free State, 2Faculty of Natural and Agricultural Sciences, Department of Plant Science, Botany, Plant Biochemistry and Physiology, University of the Free State, 3Agricultural Research Council - Small Grain Institute

The present protocol describes procedures used to study and characterize cell wall-related enzymes, mainly β-1,3-glucanase and peroxidase, in wheat plants. Their activity levels increase during wheat-RWA interaction and are involved in the plant defense response through cell wall reinforcement, which deters aphid feeding.

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