Fluorescence Staining of Cells to Study Phagocytosis
4:50
Usage of Transmission Electron Microscopy to Study Phagocytosis
7:02
Results
9:31
Conclusion
Transcript
The overall purpose of this procedure is to use microscopy as a tool to study the phagocytosis of the pathogenic fungal species Cryptococcus neoformans by its environmental predator amoeba. This video details a protocol for preparing a co-culture
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This paper details a protocol for preparing a co-culture of cryptococcal cells and amoebae that is studied using still, fluorescent images and high-resolution transmission electron microscope images. Illustrated here is how quantitative data can complement such qualitative information.