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Kindai University

3 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
Kei Miyamoto 1,2, David Simpson 1,2, John B. Gurdon 1,2
1Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, 2Department of Zoology, University of Cambridge

We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.

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Medicine

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion
Hiroki Tanaka 1,2, Naoki Unno 2, Tatsuro Yata 2, Hirona Kugo 3, Nobuhiro Zaima 3, Takeshi Sasaki 4, Tetsumei Urano 1
1Department of Medical Physiology, Hamamatsu University School of Medicine, 2Division of Vascular Surgery, Second Department of Surgery, Hamamatsu University School of Medicine, 3Department of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, 4Department of Organ & Tissue Anatomy, Hamamatsu University School of Medicine

Polyurethane catheter insertion into the aortic lumen and suture ligation of the aorta induce chronic hypoxia due to hypoperfusion of the adventitial vasa vasorum. This article describes a novel animal model of abdominal aortic aneurysm (AAA) with characteristics similar to those of AAA in humans.

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Developmental Biology

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
Rika Azuma 1, Kei Miyamoto 2, Mami Oikawa 3, Masayasu Yamada 4, Masayuki Anzai 1,5
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, 2Faculty of Biology-Oriented Science and Technology, Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, 5Institute of Advanced Technology, Kindai University

We describe a dramatically improved method for mouse cloning using trichostatin A, vitamin C, and deionized bovine serum albumin. We show a simplified, reproducible protocol that supports efficient development of cloned embryos. Hence, this method could become a standardized procedure for mouse cloning.

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