Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

12.5K Views

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09:52 min

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April 26th, 2018

DOI :

10.3791/57036-v

April 26th, 2018

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Rika Azuma¹, Kei Miyamoto², Mami Oikawa³, Masayasu Yamada⁴, Masayuki Anzai¹^,⁵

¹Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, ²Faculty of Biology-Oriented Science and Technology, Kindai University, ³Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, ⁴Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, ⁵Institute of Advanced Technology, Kindai University

Summary

Explore More Videos

Keywords Somatic Cell Nuclear Transfer

Cloning

Mouse

Embryonic Development

Nuclear Programming

Genetic Research Conservation

Cumulus Oocyte Complexes

Chapters in this video

0:05

Title

0:52

Preparation of Deionized Bovine Serum Albumin

1:48

Oocyte Collection

3:26

Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes

5:32

Fusion of Donor Cell and Enucleated Oocyte

7:42

Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C

8:26

Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment

9:15

Conclusion

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