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University of California - Davis

3 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature
Andrew J. McElrone 1,2, Brendan Choat 3, Dilworth Y. Parkinson 4, Alastair A. MacDowell 4, Craig R. Brodersen 5
1U.S. Department of Agriculture, 2Department of Viticulture and Enology, University of California - Davis, 3Hawkesbury Institute for the Environment, University of Western Sydney, 4Advanced Light Source, Lawrence Berkeley National Lab, 5Citrus Research & Education Center, University of Florida

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique that can be used to study the structure and function of plant vasculature in 3D. We demonstrate how HRCT facilitates exploration of xylem networks across a wide range of plant tissues and species.

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JoVE Journal

Surface Renewal: An Advanced Micrometeorological Method for Measuring and Processing Field-Scale Energy Flux Density Data
Andrew J. McElrone 1,2, Thomas M. Shapland 2, Arturo Calderon 2,3, Li Fitzmaurice 4,5, Kyaw Tha Paw U 4, Richard L. Snyder 4
1Crops Pathology and Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, 2Department of Viticulture & Enology, University of California, Davis, 3Faculty of Agricultural Sciences, University of Chile, 4Atmospheric Science, University of California, Davis, 5URS Corporation Australia Pty. Ltd.

Surface renewal is a micrometeorological method that is being used increasingly to determine energy fluxes, but its technical complexity makes it inaccessible to a broad audience. We describe the steps needed to set up and calibrate a surface renewal field station, to acquire and process data, and to correctly interpret results.

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Biochemistry

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Anthony Asher 1, Iniyan Ganesan 1, Laura Klasek 1, Steven M. Theg 1
1Department of Plant Biology, University of California - Davis

We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.

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