Results: Transport of Proteins Through cpTat and cpSec1 and Integration Through cpSRP Pathways
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Conclusion
Transcript
This method can help answer key questions regarding the mechanisms and energetics of protein transport across chloroplast membranes, as well as the location of proteins within plastids. The main advantage of this technique for thylakoid protein ta
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We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.