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University of Regensburg

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells
Christian Schütz 1, Martin Fleck 2,3, Jonathan P. Schneck 1, Mathias Oelke 1
1Department of Pathology, Institute of Cell Engineering, Johns Hopkins University, 2Department of Internal Medicine I, University of Regensburg, 3Department of Rheumatology, Asklepios Medical Center

Guidelines are presented for the generation of killer artificial antigen presenting cells, KaAPC, an efficient tool for in vitro depletion of human antigen-specific T cells and an alternative solution to cellular immunotherapy for the treatment of T cell-mediated autoimmune diseases in an antigen-specific fashion without compromising the remaining immune system.

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Biology

The Mouse Isolated Perfused Kidney Technique
Jan Czogalla 1, Frank Schweda 2, Johannes Loffing 1
1Institute of Anatomy, Swiss National Centre of Competence in Research Kidney, University of Zürich, 2Department of Physiology, University of Regensburg

The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.

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JoVE Core

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
Judith A. Stolwijk 1, Anne-Kathrin Mildner 1, Christian Kade 1, Michael Skiba 1, Guenther Bernhardt 2, Armin Buschauer 2, Harald Huebner 3, Peter Gmeiner 3, Joachim Wegener 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.

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Engineering

Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios
Burkhard Hoppenstedt 1, Thomas Probst 2, Manfred Reichert 1, Winfried Schlee 3, Klaus Kammerer 1, Myra Spiliopoulou 4, Johannes Schobel 1, Michael Winter 1, Anna Felnhofer 5, Oswald D. Kothgassner 6, Rüdiger Pryss 7
1Institute of Databases and Information Systems, Ulm University, 2Department for Psychotherapy and Biopsychosocial Health, Danube University Krems, 3Department of Psychiatry and Psychotherapy, University of Regensburg, 4Faculty of Computer Science, Otto von Guericke University Magdeburg, 5Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 6Department of Child and Adolescent Psychiatry, Medical University of Vienna, 7Institute of Clinical Epidemiology and Biometry, University of Würzburg

This protocol delineates the technical setting of a developed mixed reality application that is used for immersive analytics. Based on this, measures are presented, which were used in a study to gain insights into usability aspects of the developed technical solution.

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