JoVE Journal

Immunology and Infection

This content is Open Access.

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

Guidelines are presented for the generation of killer artificial antigen presenting cells, KaAPC, an efficient tool for in vitro depletion of human antigen-specific T cells and an alternative solution to cellular immunotherapy for the treatment of T cell-mediated autoimmune diseases in an antigen-specific fashion without compromising the remaining immune system.

Chapters in this video

0:05

Title

1:32

Generation of HLA-A2-Ig Based Killer Artificial Antigen Presenting Cells (KaAPC)

3:19

Quality Control, Peptide Loading, Washing, Storage, and Stability of KaAPC

4:53

KaAPC In Vitro Killing Assay

6:23

Results: KaAPC Eliminate CTL in an Antigen-specific Fashion

7:43

Conclusion

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