University of Iceland

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein

Stefán R. Jónsson¹, Valgerdur Andrésdóttir¹

¹Institute for Experimental Pathology, University of Iceland

We describe a molecular clone of maedi-visna virus that expresses GFP and is fully infectious. Replication of this virus can be detected by using fluorescence microscopy and flow cytometry.

Biochemistry

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Chase M. Clark¹, Maria S. Costa^1,2, Erin Conley¹, Emma Li¹, Laura M. Sanchez¹, Brian T. Murphy¹

¹Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, ²Faculty of Pharmaceutical Sciences, University of Iceland

IDBac is an open-source mass spectrometry-based bioinformatics pipeline that integrates data from both intact protein and specialized metabolite spectra, collected on cell material scraped from bacterial colonies. The pipeline allows researchers to rapidly organize hundreds to thousands of bacterial colonies into putative taxonomic groups, and further differentiate them based on specialized metabolite production.

Medicine

Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images

Andrea García-Llorca¹, Hallur Reynisson¹, Thor Eysteinsson^1,2

¹Department of Physiology, Biomedical Center, Faculty of Medicine, University of Iceland, ²Department of Ophthalmology, National University Hospital

Mouse retinal vasculature is particularly interesting in understanding the mechanisms of vascular pattern formation. This protocol automatically measures the diameter of mouse retinal vessels from fluorescent angiography fundus images at a fixed distance from the optic disk.