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South China University of Technology

16 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
Mianbo Huang 1, Jin Xu 1, Chong Hyun Shin 1
1School of Biology, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology

Sustained fibrosis with deposition of excessive extracellular matrix proteins leads to cirrhosis. Alcohol abuse is one of the main causes of severe liver disease. We established an ethanol-induced zebrafish fibrotic liver model to study the mechanisms and strategies of promoting hepatocyte regeneration upon alcohol-induced injury.

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Chemistry

Green and Low-cost Production of Thermally Stable and Carboxylated Cellulose Nanocrystals and Nanofibrils Using Highly Recyclable Dicarboxylic Acids
Huiyang Bian 1,3, Liheng Chen 2,3, Ruibin Wang 2,3, Junyong Zhu 3
1Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, 2State Key Lab of Pulp and Paper Engineering, South China University of Technology, 3Forest Products Laboratory, U.S. Forest Service, U.S. Department of Agriculture

Here we demonstrate a novel method for green and sustainable productions of highly thermally stable and carboxylated cellulose nanocrystals (CNC) and nanofibrils (CNF) using highly recyclable solid dicarboxylic acids.

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Bioengineering

A Performance-testing Platform for a Conduction Micropump with an FR-4 Copper-clad Electrode Plate
Junyuan Feng 1, Zhenping Wan 1, Chen Feng 1, Wanyu Wen 1, Yong Tang 1
1School of Mechanical and Automotive Engineering, South China University of Technology

This paper presents a protocol for the fabrication of a conduction micropump using symmetric planar electrodes on flame-retardant glass-reinforced epoxy (FR-4) copper-clad laminate (CCL) to test the influence of chamber dimensions on the performance of a conduction micropump.

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Study on the Moisture Absorption and Desorption Rate of Bamboo Scrimber in a Hot-Humid Climatic Wind Tunnel
Zujian Huang 1,2,3, Yimin Sun 1,2, Florian Musso 3
1School of Architecture, South China University of Technology, 2State Key Laboratory of Subtropical Building Science, 3Chair of Building Construction and Material Science, Department of Architecture, Technical University of Munich

Here, we present a protocol to measure the moisture absorption and desorption rate of bamboo scrimber in an outdoor environment for a dynamic hot-humid climate wind tunnel test with complete meteorological conditions for 72 h.

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Immunology and Infection

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
Ming Fu 1, Zefeng Lin 2, Huiting Lin 2, Yanlu Tong 2, Hezhen Wang 2, Hongjiao Chen 2, Yan Chen 2, Ruizhong Zhang 2
1First Affiliated Hospital of Jinan University, 2Department of Pediatric Surgery, Guangzhou Women & Children's Medical Center, Guangzhou Medical University

This article describes in detail a method based on silver nanoparticles for ameliorating biliary atresia syndrome in an experimental biliary atresia mouse model. A solid understanding of the reagent preparation process and the neonatal mouse injection technique will help familiarize researchers with the method used in neonatal mouse model studies.

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Chemistry

Stereolithographic 3D Printing with Renewable Acrylates
Vincent S.D. Voet 1, Geraldine H.M. Schnelting 1, Jin Xu 2, Katja Loos 2, Rudy Folkersma 1, Jan Jager 1
1Professorship Sustainable Polymers, NHL Stenden University of Applied Sciences, 2Macromolecular Chemistry and New Polymeric Materials, Zernike Institute for Advanced Materials, University of Groningen

A protocol for additive manufacturing with renewable photopolymer resins on a stereolithography apparatus is presented.

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Immunology and Infection

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
Raghubendra Singh Dagur 1, Weimin Wang 1, Edward Makarov 1, Yimin Sun 2, Larisa Y. Poluektova 1
1Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 2Department of Pathology and Microbiology, University of Nebraska Medical Center

This protocol provides a reliable method to establish humanized mice with both human immune system and liver cells. Dual reconstituted immunodeficient mice achieved via intrasplenic injection of human hepatocytes and CD34+ hematopoietic stem cells are susceptible to human immunodeficiency virus-1 infection and recapitulate liver damage as observed in HIV-infected patients.

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Neuroscience

Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression
Weipeng Zheng *1, Xiuhang Ruan *2, Xinhua Wei 3, Fangtian Xu 4, Yuanping Huang 1, Ning Wang 1, Haoyi Chen 5, YingJie Liang 6, Wende Xiao 6, Xin Jiang 1, Shifeng Wen 6
1Department of Orthopedics, Guangzhou First People's Hospital, Guangzhou Medical University, 2Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, 3Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, 4Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, 5Department of Orthopedics, Guangzhou Chest Hospital, 6Department of Orthopedics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou Medical University

Here, we present a protocol for the application of diffusion tensor imaging parameters to evaluate spinal cord compression.

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Chemistry

Synthesis of Graphene Nanofluids with Controllable Flake Size Distributions
Du Baolei 1, Jian Qifei 1
1Department of Mechanical and Automotive Engineering, South China University of Technology

A method for synthesizing graphene nanofluids with controllable flake size distributions is presented.

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Developmental Biology

An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
Yunyun Jiang 1, Jiahao Chen 1, Jin Xu 2
1Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration.

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Medicine

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
Xiaofan Jia *1,2, Ling He *1,3, Yong Gu 1, Hilary High 1, Liping Yu 1
1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, 2Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, 3Department of endocrinology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology

We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1.

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Neuroscience

Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity
Qiangqiang Wang *1, Ju Zhang *2, Yiyi Jiang 1,3, Yue Xiao 1, Xiaomin Li 3, Xinliang Mao 3, Zebo Huang 1
1Institute for Food Nutrition and Human Health, School of Food Science and Engineering, South China University of Technology, 2School of Bioscience and Bioengineering, Hebei University of Economics and Business, 3Perfect Life & Health Institute

Here, we present a protocol to assess the neuroprotective activities of test compounds in Caenorhabditis elegans, including polyglutamine aggregation, neuronal death, and chemoavoidance behavior, as well as an exemplary integration of multiple phenotypes.

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Medicine

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis
Yan Tian *1, Junyu Huang *1, Ming Fu 1, Qiuming He 1, Jiale Chen 1, Yan Chen 1, Ruizhong Zhang 1, Wei Zhong 1
1Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University

Necrotizing enterocolitis (NEC) is the most severe gastrointestinal (GI) disease that often occurs in premature infants, especially very low birth weight infants, with high mortality and unclear pathogenesis. The cause of NEC may be related to inflammatory immune regulatory system abnormalities. An NEC animal model is an indispensable tool for NEC disease immune research. NEC animal models usually use C57BL/6J neonatal mice; BALB/c neonatal mice are rarely used. Related studies have shown that when mice are infected, Th2 cell differentiation is predominant in BALB/c mice compared to C57BL/6J mice. Studies have suggested that the occurrence and development of NEC are associated with an increase in T helper type 2 (Th2) cells and are generally accompanied by infection. Therefore, this study used neonatal BALB/c mice to induce an NEC model with similar clinical characteristics and intestinal pathological changes as those observed in children with NEC. Further study is warranted to determine whether this animal model could be used to study Th2 cell responses in NEC.

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Immunology and Infection

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
Xiaofan Jia *1, Ling He *1,2, Dongmei Miao 1, Caiguo Zhang 1, Marian Rewers 1, Liping Yu 1
1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, 2Department of Endocrinology, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology

In line with the urgent need for screening for type 1 diabetes, celiac disease, and coronavirus disease 2019, we developed a high throughput 6-Plex electrochemiluminescence assay to simultaneously detect all four islet autoantibodies, tissue transglutaminase autoantibodies, and antibodies to the receptor binding domain of severe acute respiratory syndrome coronavirus 2.

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Immunology and Infection

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia
Xinrui Wang 1,2, Ruizhong Zhang 2, Zefeng Lin 2, Ming Fu 2, Yan Chen 1,2
1School of Medicine, South China University of Technology, 2Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery, Guangzhou Women and Children’s Medical Center

We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments.

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Medicine

Cerebral Ischemic Coma Model Induced by Modified Four-Vessel Occlusion
Rong Ma *1,2, Rui Li *2, Jianlong Liang 2, Hongyan Yang 2, Qian Xie 2, Xuxin Zeng 2, Jialiang Guo 2
1School of Chemistry and Chemical Engineering, South China University of Technology, 2School of Medicine, Foshan University

This protocol describes the process for inducing a cerebral ischemic coma model using a modified four-vessel occlusion method.

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