Isolation and Culture of Human Fungiform Taste Papillae CellsHakan Ozdener 1, Andrew I. Spielman 2, Nancy E. Rawson 3
1Monell Chemical Senses Center, 2New York University College of Dentistry, 3AFB International
We aimed to develop a reproducible protocol for isolating and maintaining long-term cultures of human fungiform taste papillae cells. Cells from human fungiform papillae obtained by biopsy were successfully maintained in culture for more than eight passages (12 months) without loss of viability.