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Hiroshima University

6 ARTICLES PUBLISHED IN JoVE

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Environment

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Megumi Kuroiwa 1, Keitaro Fukushima 2,3, Kazuma Hashimoto 2, Yukiko Senga 4, Tsubasa Sato 4, Chie Katsuyama 5, Yuichi Suwa 1
1Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 2Faculty & Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 3Center for Ecological Research, Kyoto University, 4Department of Chemistry, Faculty of Science, Toho University, 5Graduate School of Integrated Arts and Sciences, Hiroshima University

A series of methods to determine the potential DNRA rate based on 14NH4+/15NH4+ analyses is provided in detail. NH4+ is converted into N2O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.

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Environment

Removal of Arsenic Using a Cationic Polymer Gel Impregnated with Iron Hydroxide
Syed Ragib Safi 1, Takehiko Gotoh 1, Takashi Iizawa 1, Satoshi Nakai 1
1Department of Chemical Engineering, Hiroshima University

In this work, we prepared an adsorbent composed of the cationic N,N-dimethylamino propylacrylamide methyl chloride quaternary (DMAPAAQ) polymer gel and iron hydroxide for adsorbing arsenic from groundwater. The gel was prepared via a novel method designed to ensure the maximum content of iron particles in its structure.

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Behavior

Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration
Kazuki Shigyou *1, Haruka Maeoka *1, Ryuji Igarashi 2,3,4, Takuma Sugi 1
1Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University, 2Institute for Quantum Life Science, National Institute for Quantum and Radiological Science and Technology, 3National Institute for Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, 4JST, PRESTO

Reported here is a system for calcium imaging in freely behaving Caenorhabditis elegans with well-controlled, nonlocalized vibration. This system allows researchers to evoke nonlocalized vibrations with well-controlled properties at nano-scale displacement and to quantify calcium currents during responses of C. elegans to the vibrations.

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Environment

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis
Kyoko Yarimizu 1, So Fujiyoshi 1, Mikihiko Kawai 2, Jacquelinne J. Acuña 3, Joaquin-Ignacio Rilling 3, Marco Campos 3, Jonnathan Vilugrón 4, Henry Cameron 5, Karen Vergara 6, Gonzalo Gajardo 6, Oscar Espinoza-González 4, Leonardo Guzmán 4, Satoshi Nagai 7, Carlos Riquelme 5, Milko A. Jorquera 3, Fumito Maruyama 1
1Office of Research and Academia-Government-Community Collaboration, Hiroshima University, 2Graduate School of Human and Environmental Studies, Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus, Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas, Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency, Fisheries Resources Institute

This protocol introduces steps of metabarcoding analysis, targeting 16S rRNA and 18S rRNA genes, for monitoring harmful algal blooms and their associated microbiome in seawater samples. It is a powerful molecular-based tool but requires several procedures, which are visually explained here step-by-step.

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Biology

The Large-Scale Cultivation of Nematodes to Study Their Collective Behaviors
Ryuki Imamura *1, Yurina Nakane *1, Hu Jiajing 1, Hiroshi Ito 2, Takuma Sugi 1
1Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University, 2Faculty of Design, Kyushu University

Here, a system is reported for studying the collective behaviors of nematodes by culturing them in bulk using dog food agar medium. This system allows researchers to propagate large numbers of dauer worms and can be applied to Caenorhabditis elegans and other related species.

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Biology

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
Hiroko Shinjo 1, Gaku Nagano 1, Shogo Ishii 1, Natsumi Himeno 1, Yutaro Yamamoto 1, Junji Sagawa 1, Ryuta Baba 1, Gentaro Egusa 1, Noboru Hattori 1, Haruya Ohno 1
1Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences, Hiroshima University

Protein-protein interactions are important for elucidating the function of target proteins, and co-immunoprecipitation (co-IP) can easily confirm PPIs. We transiently transfected a plasmid encoding an epitope-tagged protein into HEK-293 cells and developed an immunoprecipitation method to easily confirm the binding of two target proteins.

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