Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

1.1K Views

07:16 min

January 5th, 2024

DOI :

10.3791/66085-v

January 5th, 2024


Transcript

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Immunoprecipitation

Chapters in this video

0:00

Introduction

1:50

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

3:02

Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel

4:17

Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein‐Protein Interaction Studies

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