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Medical University of Warsaw

4 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture
Adrianna Maria Piasek *1, Iryna Levkovych *1, Paulina Musolf 1, Hanna Chmielewska 1, Aneta Ścieżyńska 2, Anna Sobiepanek 1
1Chair of Drug and Cosmetics Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 2Department of Histology and Embryology, Medical University of Warsaw

Here, we present inexpensive and simple procedures to introduce various 3D skin models for routine research in a cell culture laboratory. Researchers can create models tailored to their needs without relying on commercially available models.

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Neuroscience

Motion-Acuity Test for Visual Field Acuity Measurement with Motion-Defined Shapes
Marco Ninghetto 1, Michał Wieteska 2,3, Anna Kozak 1, Kamil Szulborski 4, Tomasz Gałecki 4, Jacek Szaflik 4, Kalina Burnat 1
1Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Mossakowski Medical Research Institute, Polish Academy of Sciences, 3Institute of Radio Electronics and Multimedia Technology, Warsaw University of Technology, 4Department of Ophthalmology, Medical University of Warsaw

A novel motion-based acuity test that allows the assessment of central and peripheral visual processing in low-vision and healthy individuals, along with goggles limiting peripheral vision compatible with MRI protocols, are described here. This method offers a comprehensive vision assessment for functional impairments and dysfunctions of the visual system.

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Medicine

Preparation and Analysis of Histological Slides of Rat and Mouse Eyeballs to Evaluate the Retina
Ewa Sikorska *1, Dominika Wołosz *2, Kaja Kasarełło 1, Łukasz Koperski 2, Barbara Górnicka 2, Agnieszka Cudnoch-Jędrzejewska 1
1Chair and Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, 2Department of Pathology, Medical University of Warsaw

This manuscript presents a protocol for the histological preparation of slides from rodent eyeballs. With the proposed method, the inner retina can be easily visualized and assessed under a light microscope. The procedure is presented for the eyeballs of mice and rats.

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