Procedure 1: Insertion of the Line for Intraintestinal Administration
3:57
Procedure 2: Inferior Vena Cava Catheterization
6:44
Procedure 3: Portal Vein Catheterization
10:46
Procedure 4: Portal Vein Blood Sampling
11:22
Procedure 5: Inferior Vena Cava Blood Sampling
11:51
Procedure 6: Administration of Permeability Marker
12:59
Procedure 7: Calculation of Hepatic Clearance
13:20
Results
14:16
Conclusion
Transcript
The gut-blood barrier is a multilayer system that controls the passage of nutrients, bacterial metabolites, drugs, and other exogenous compounds from intestinal lumen to the blood stream. As we know, the integrity of the barrier may be impaired in
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The access of nutrients, microbiota metabolites and medicines to the circulation is controlled by the gut-blood barrier (GBB). We describe a direct method for measuring the GBB permeability in vivo, which, in contrast to commonly used indirect methods, is virtually not affected by liver and kidney functions.