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Universitat Autònoma de Barcelona

8 ARTICLES PUBLISHED IN JoVE

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Environment

How to Create Conditioned Taste Aversion for Grazing Ground Covers in Woody Crops with Small Ruminants
Carmen L. Manuelian 1, Elena Albanell 1, Maristela Rovai 1,2, Gerardo Caja 1
1Group of Ruminant Research (G2R), Department of Animal and Food Science, Universitat Autònoma de Barcelona, 2Dairy Science Department, South Dakota State University

Feeding behavior preferences in livestock can be modified to implement a grazing management plan in woody crops. Here, we present a protocol to show a lithium chloride dose after eating a new plant that induces conditioned taste aversion.

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Developmental Biology

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Marta Vila-Cejudo 1, Elena Ibañez 1, Josep Santalo 1
1Unitat de Biologia Cellular, Facultat de Biociències, Universitat Autònoma de Barcelona

This article describes a protocol to efficiently derive and culture pluripotent stem cell lines from mouse embryos at the blastocyst stage.

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Biochemistry

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
Anita Carija 1, Salvador Ventura 1, Susanna Navarro 1
1Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona

Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide.

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Immunology and Infection

Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds
Jie Ji 1,2, Rosemary Thwaite 1,2, Nerea Roher 1,2
1Institute of Biotechnology and Biomedicine (IBB-UAB), Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona

The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.

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JoVE Core

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
Aida Llucià-Valldeperas 1,2, Ramon Bragós 3, Antoni Bayés-Genís 1,4,5,6
1Insuficiencia Cardiaca y Regeneración Cardiaca (ICREC) Research Program, Health Science Research Institute Germans Trias i Pujol, 2Amsterdam Universitair Medisch Centrum (UMC), Vrije Universiteit Amsterdam, Pulmonology and Physiology, Amsterdam Cardiovascular Sciences, 3Electronic and Biomedical Instrumentation Group, Departament d'Enginyeria Electrònica, Universitat Politècnica de Catalunya, 4Cardiology Service, Germans Trias i Pujol University Hospital, 5Department of Medicine, Universitat Autònoma de Barcelona, 6Centro de Investigación Biomédica en Red (CIBER) Cardiovascular, Instituto de Salud Carlos III

Here we present a protocol for training a cell population using electrical and mechanical stimuli emulating cardiac physiology. This electromechanical stimulation enhances the cardiomyogenic potential of the treated cells and is a promising strategy for further cell therapy, disease modeling, and drug screening.

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Cancer Research

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
Joan Repullés 1,2, Mariona Terradas 1,3,4, Gemma Fuster 5,6,7, Anna Genescà 1, Teresa Anglada 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described.

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JoVE Journal

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
Sandra Guallar-Garrido 1, Marina Luquin 1, Esther Julián 1
1Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona

A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.

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Neuroscience

Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma
Natalia Valle-Tamayo 1,2, Rocío Pérez-González 2,3, Gemma Chiva-Blanch 4,5, Olivia Belbin 1,2, Sara Serrano-Requena 1,2, Sònia Sirisi 1,2, Alba Cervantes González 1,2, Oriol Giró 4,5, Érika Sánchez-Aced 1,2, Oriol Dols-Icardo 1,2, Daniel Alcolea 1,2, María Carmona-Iragui 1,2, Amanda Jimenez 4,5, Alberto Lleó 1,2, Juan Fortea 1,2, M. Florencia Iulita 1,2
1Sant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau, Universitat Autònoma de Barcelona, 2Center of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED), 3Movement Disorders Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, 4Department of Endocrinology and Nutrition, Hospital Clínic de Barcelona, August Pi i Sunyer Biomedical Research Institute - IDIBAPS, 5Spanish Biomedical Research Network in Physiopathology of Obesity and Nutrition (CIBEROBN)

This protocol describes the enrichment of astrocyte-derived extracellular vesicles (ADEVs) from human plasma. It is based on the separation of EVs by polymer precipitation, followed by ACSA-1 based immunocapture of ADEVs. Analysis of ADEVs may offer clues to study changes in inflammatory pathways of living patients, non-invasively by liquid biopsy.

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