Harvard Medical School and Howard Hughes Medical Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Claudio Vinegoni¹, Daniel Razansky², Chrysoula Pitsouli³, Norbert Perrimon³, Vasilis Ntziachristos², Ralph Weissleder¹

¹Center for Systems Biology, Massachusetts General Hospital, ²Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich, ³Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute

Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.

Biology

Primary Cell Cultures from Drosophila Gastrula Embryos

Norbert Perrimon^1,2, Jonathan Zirin¹, Jianwu Bai¹

¹Department of Genetics, Harvard Medical School, ²Howard Hughes Medical Institute

We provide a detailed protocol for preparing primary cells dissociated from Drosophila embryos. The ability to carry out the effective RNAi perturbation, together with other molecular, biochemical and cell imaging methods will allow a variety of questions to be addressed in Drosophila primary cells.