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Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

10.6K Views

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11:51 min

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August 20th, 2009

DOI :

10.3791/1510-v

August 20th, 2009

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1Center for Systems Biology, Massachusetts General Hospital, 2Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich, 3Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute

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Mesoscopic Fluorescence Tomography
In vivo Imaging
Developing Drosophila
Optical Interrogation
Molecular Changes
Functional Changes
Optical Imaging Methods
Penetration Limits
Optical Microscopy
Diffusion imposed Limits
Optical Macroscopy
Model Organisms
Insects
Animal Embryos
Small Animal Extremities
Nanometer resolution Optical Imaging
Imaging Penetration Depth
Photon Scattering
Tissues
Entire Embryos Imaging
Chemical Treatment
Scattering Clearance
Post mortem Imaging
Two photon Microscopy
Confocal Microscopy
Developmental Biology
Drug Discovery

Chapters in this video

0:05

Title

0:54

Introduction

1:34

Preparation of Drosophila

4:13

Mounting Drosophila Pupae for Imaging

5:16

Scanning a Tissue of Interest

6:18

Time-lapse Imaging

7:58

Three Dimensional Reconstruction of Fluorescence

9:59

Representative Results/ Outcome

10:58

Conclusion

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