University of Maryland Baltimore County

4 ARTICLES PUBLISHED IN JoVE

Biology

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization

David Dunston¹, Sarah Ashby^*1, Kurt Krosnowski^*1, Tatsuya Ogura¹, Weihong Lin¹

¹Biological Sciences, University of Maryland Baltimore County

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization

Developmental Biology

Upright Imaging of Drosophila Egg Chambers

Lathiena Manning¹, Michelle Starz-Gaiano¹

¹Department of Biological Sciences, University of Maryland Baltimore County

The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.

Biology

Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

Shonda M. Campbell¹, Michelle Starz-Gaiano¹, Jeff Leips¹

¹Department of Biology, University of Maryland Baltimore County

This protocol describes an in vivo assay of phagocytosis used to assess and quantify the ability of young and aged Drosophila melanogaster hemocytes to phagocytose bacteria.

Biochemistry

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

Danté Johnson¹, Benjamin Punshon-Smith², Jessica A. Espino¹, Anne Gershenson³, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences, University of Maryland Baltimore, ²Technology Research Center, University of Maryland Baltimore County, ³Department of Biochemistry and Molecular Biology, University of Massachusetts

A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP).