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University of Maryland Baltimore County

4 ARTICLES PUBLISHED IN JoVE

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Biology

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization
David Dunston 1, Sarah Ashby *1, Kurt Krosnowski *1, Tatsuya Ogura 1, Weihong Lin 1
1Biological Sciences, University of Maryland Baltimore County

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization

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Developmental Biology

Upright Imaging of Drosophila Egg Chambers
Lathiena Manning 1, Michelle Starz-Gaiano 1
1Department of Biological Sciences, University of Maryland Baltimore County

The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.

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Biology

Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay
Shonda M. Campbell 1, Michelle Starz-Gaiano 1, Jeff Leips 1
1Department of Biology, University of Maryland Baltimore County

This protocol describes an in vivo assay of phagocytosis used to assess and quantify the ability of young and aged Drosophila melanogaster hemocytes to phagocytose bacteria.

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Biochemistry

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
Danté Johnson 1, Benjamin Punshon-Smith 2, Jessica A. Espino 1, Anne Gershenson 3, Lisa M. Jones 1
1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2Technology Research Center, University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology, University of Massachusetts

A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP).

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