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Universidad Autónoma de Querétaro

4 ARTICLES PUBLISHED IN JoVE

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Biology

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Ivanna Karina Olivares-Marin 1, Juan Carlos González-Hernández 2, Carlos Regalado-Gonzalez 1, Luis Alberto Madrigal-Perez 3
1Department of Chemistry, Universidad Autónoma de Querétaro, 2Department of Biochemical Engineering, Instituto Tecnológico de Morelia, 3Department of Biochemical Engineering, Instituto Tecnológico Superior de Ciudad Hidalgo

Here we present a protocol to estimate the respiratory and fermentative metabolism by fitting the exponential growth of Saccharomyces cerevisiae to the exponential growth equation. Calculation of the kinetic parameters allows for the screening of influences of substances/compounds on fermentation or mitochondrial respiration.

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Immunology and Infection

Quantitation of Rabies Virus in Various Bovine Brain Structures
Edith Rojas-Anaya 1, Daniela Anaya-Razo 2, Isabel Bárcenas-Reyes 3, Elizabeth Loza-Rubio 1
1Centro Nacional de Investigación Disciplinaria en Microbiología Animal (CENID-MA), INIFAP, 2Campus Toluca, Universidad del Valle de México, 3Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro

This protocol presents a qRT-PCR-based approach for determining the rabies virus nucleoprotein (N) gene copy number within various bovine brain anatomical structures using in vitro transcription.

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Environment

Using Tree-Rings to Reconstruct Fire History Information from Forested Areas
Julián Cerano-Paredes 1, Jose M. Iniguez 2, José Villanueva-Díaz 1, Rosalinda Cervantes-Martínez 1, Víctor H. Cambrón-Sandoval 3, Josué R. Estrada-Arellano 4, Gerardo Esquivel-Arriaga 1, Osvaldo Franco-Ramos 5, Lorenzo Vázquez-Selem 5, Gabriel F. Cardoza-Martínez 4
1CENID-RASPA, INIFAP, 2Rocky Mountain Research Station, USDA Forest Service, 3Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, 4Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, 5Instituto de Geografía, Universidad Nacional Autónoma de México

This work explains the most appropriate techniques and methods for conducting a fire history study from beginning site selection to final analysis of fire-climate relationship.

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Environment

How to Extract Climate Variability from Tree-Rings
Julián Cerano-Paredes 1, Paul Szejner 2, Genaro Gutiérrez-García 2, Rosalinda Cervantes-Martínez 1, Víctor H. Cambrón-Sandoval 3, José Villanueva-Díaz 1, Josué R. Estrada-Arellano 4, Osvaldo Franco-Ramos 5, Lorenzo Vázquez-Selem 5, Luis U. Castruita-Esparza 6
1INIFAP, CENID-RASPA, 2Instituto de Geología, Universidad Nacional Autónoma de México, 3Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, 4Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, 5Instituto de Geografía, Universidad Nacional Autónoma de México, 6Facultad de Ciencias Agrícolas y Forestales, Universidad Autónoma de Chihuahua

Tree-ring climate reconstructions can be helpful to better understand past climate variability beyond instrumental records. This protocol shows how to reconstruct past climate using tree rings and meteorological instrumental records.

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