Universidad Autónoma de Querétaro

5 ARTICLES PUBLISHED IN JoVE

Biology

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Ivanna Karina Olivares-Marin¹, Juan Carlos González-Hernández², Carlos Regalado-Gonzalez¹, Luis Alberto Madrigal-Perez³

¹Department of Chemistry, Universidad Autónoma de Querétaro, ²Department of Biochemical Engineering, Instituto Tecnológico de Morelia, ³Department of Biochemical Engineering, Instituto Tecnológico Superior de Ciudad Hidalgo

Here we present a protocol to estimate the respiratory and fermentative metabolism by fitting the exponential growth of Saccharomyces cerevisiae to the exponential growth equation. Calculation of the kinetic parameters allows for the screening of influences of substances/compounds on fermentation or mitochondrial respiration.

Immunology and Infection

Quantitation of Rabies Virus in Various Bovine Brain Structures

Edith Rojas-Anaya¹, Daniela Anaya-Razo², Isabel Bárcenas-Reyes³, Elizabeth Loza-Rubio¹

¹Centro Nacional de Investigación Disciplinaria en Microbiología Animal (CENID-MA), INIFAP, ²Campus Toluca, Universidad del Valle de México, ³Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro

This protocol presents a qRT-PCR-based approach for determining the rabies virus nucleoprotein (N) gene copy number within various bovine brain anatomical structures using in vitro transcription.

Environment

Using Tree-Rings to Reconstruct Fire History Information from Forested Areas

Julián Cerano-Paredes¹, Jose M. Iniguez², José Villanueva-Díaz¹, Rosalinda Cervantes-Martínez¹, Víctor H. Cambrón-Sandoval³, Josué R. Estrada-Arellano⁴, Gerardo Esquivel-Arriaga¹, Osvaldo Franco-Ramos⁵, Lorenzo Vázquez-Selem⁵, Gabriel F. Cardoza-Martínez⁴

¹CENID-RASPA, INIFAP, ²Rocky Mountain Research Station, USDA Forest Service, ³Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, ⁴Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, ⁵Instituto de Geografía, Universidad Nacional Autónoma de México

This work explains the most appropriate techniques and methods for conducting a fire history study from beginning site selection to final analysis of fire-climate relationship.

Environment

How to Extract Climate Variability from Tree-Rings

Julián Cerano-Paredes¹, Paul Szejner², Genaro Gutiérrez-García², Rosalinda Cervantes-Martínez¹, Víctor H. Cambrón-Sandoval³, José Villanueva-Díaz¹, Josué R. Estrada-Arellano⁴, Osvaldo Franco-Ramos⁵, Lorenzo Vázquez-Selem⁵, Luis U. Castruita-Esparza⁶

¹INIFAP, CENID-RASPA, ²Instituto de Geología, Universidad Nacional Autónoma de México, ³Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, ⁴Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, ⁵Instituto de Geografía, Universidad Nacional Autónoma de México, ⁶Facultad de Ciencias Agrícolas y Forestales, Universidad Autónoma de Chihuahua

Tree-ring climate reconstructions can be helpful to better understand past climate variability beyond instrumental records. This protocol shows how to reconstruct past climate using tree rings and meteorological instrumental records.

Biochemistry

Mitochondrial Respiration Quantification in Yeast Whole Cells

Gerardo M. Nava¹, Andres Carrillo-Garmendia¹, Juan Carlos González-Hernández², Luis Alberto Madrigal-Perez³

¹School of Chemistry, Universidad Autónoma de Querétaro, ²Department of Biochemical Engineering, Tecnológico Nacional de México/Instituto Tecnológico de Morelia, ³Department of Biochemical Engineering, Tecnológico Nacional de México/Instituto Tecnológico Superior de Ciudad Hidalgo

Mitochondrial respiration in yeast whole cells is a valuable indicator of cell bioenergetics. Here, we present a protocol to quantify this phenotype applicable to different yeast species.