Metabolic Labeling and Profiling of Transfer RNAs Using MacroarraysSophia Emetu *1, Morgan Troiano *1, Jacob Goldmintz *1, Jensen Tomberlin 1, Simon Grelet 2, Philip H. Howe 2, Christopher Korey 3, Renaud Geslain 1
1Laboratory of tRNA Biology, Department of Biology, College of Charleston, 2Department of Biochemistry and Molecular Biology, MUSC, 3Department of Biology, College of Charleston
We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.
