January 16th, 2018
•We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.
Tags
Related Videos
In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
Methodology for Accurate Detection of Mitochondrial DNA Methylation
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Identification of Circular RNAs using RNA Sequencing
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved